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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Methods. 2013 May 23;63(2):101–109. doi: 10.1016/j.ymeth.2013.05.007

Figure 3. The OshR platform ensures proper Guide strand production.

Figure 3

A) Current shRNA designs can display variability in specifying the accumulation of both the Guide strand and Passenger strand. B) The Organic shRNA design builds in mismatches to direct specific Drosha and Dicer cleavage sites. C) Secondary structures of the OshR 2A and 6C against the X.tropicalis Tyrosinase (Tyr) mRNA. Guide strand is in red, Passenger strand is in blue, and intentional mismatched bases are underlined and uppercase. D) Northern blots indicate OshR designs yield predominant accumulation of the Guide Strand over Passenger strand. Mock is a mock transfection; OshR is the shRNA expression vector transfection; P.ctrl: synthetic Passenger strand mimic; G.ctrl: synthetic Guide strand mimic. Red dashed rectangles mark lanes of shRNA production.