Skip to main content
. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Methods. 2013 May 23;63(2):101–109. doi: 10.1016/j.ymeth.2013.05.007

Figure 4. Gene silencing efficacy of various organic shRNAs.

Figure 4

A) Diagram of OshR target sites against mouse NEK-2 (top) and Western blots showing knockdown of Myc-tagged NEK2 (myc-NEK2) by all three OshRNAs (bottom). Actin is a loading control. Myc-NEK2 rescue constructs with mismatched bases to the shRNAs are resistant to knockdown by the OshRs. B) Diagram of OshR target sites against Xenopus Tyr (top) and Western blots showing knockdown of FLAG-tagged Tyr by OshRs marked by dashed rectangles (bottom). Tubulin is a loading control and GFP indicates proper expression of the OshR vector.