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. Author manuscript; available in PMC: 2014 Mar 26.

Published in final edited form as: J Immunotoxicol. 2010 Aug 24;7(4):298–307. doi: 10.3109/1547691X.2010.509289

Effects of WTC_2.5 on IL-6 formation by BEAS-2B cells. (A) BEAS-2B cells were treated with varying doses of WTC_2.5 (in 0.5 ml total volumes) for 5 hr, and then a reverse transcription polymerase chain reaction was used to assess IL-6 gene expression in the cells. Here, 100 and 200 μg WTC_2.5/ml are equivalent to about 5 and 10 μg WTC_2.5/cm2, respectively. Expression of β-actin was analyzed in parallel to ensure equal amounts of cDNA for each sample. (B) Corresponding IL-6 release in the BEAS-2B culture medium following the WTC_2.5 treatments as in (A). BEAS-2B cells were exposed to WTC_2.5 as in (A), and the IL-6 level in culture media was measured using ELISA. (C) BEAS-2B cells were treated by 100 μg/ml WTC_2.5 or TiO₂ for 5 hr (N = 3), and the levels of IL-6 in the cell media were measured by ELISA. *Indicates a statistically significant change (p < 0.05) when compared to value for untreated samples. Values shown are the mean relative level vs. that in unexposed control cultures (± SD) from four replicate cultures.

Effects of WTC_2.5 on IL-6 formation by BEAS-2B cells. (A) BEAS-2B cells were treated with varying doses of WTC_2.5 (in 0.5 ml total volumes) for 5 hr, and then a reverse transcription polymerase chain reaction was used to assess IL-6 gene expression in the cells. Here, 100 and 200 μg WTC_2.5/ml are equivalent to about 5 and 10 μg WTC_2.5/cm2, respectively. Expression of β-actin was analyzed in parallel to ensure equal amounts of cDNA for each sample. (B) Corresponding IL-6 release in the BEAS-2B culture medium following the WTC_2.5 treatments as in (A). BEAS-2B cells were exposed to WTC_2.5 as in (A), and the IL-6 level in culture media was measured using ELISA. (C) BEAS-2B cells were treated by 100 μg/ml WTC_2.5 or TiO₂ for 5 hr (N = 3), and the levels of IL-6 in the cell media were measured by ELISA. *Indicates a statistically significant change (p < 0.05) when compared to value for untreated samples. Values shown are the mean relative level vs. that in unexposed control cultures (± SD) from four replicate cultures.