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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Biomater Sci. 2013 Oct 9;2(2):156–166. doi: 10.1039/C3BM60142K

Fig.2.

Fig.2

Characterization of NP-EGF conjugates. (a-c) TEM image of NP-EGF conjugates after incubation with biotinylated anti-EGF antibodies and Neutravidin and subsequent labeling with biotinylated 5 nm Au NPs. (d-f) TEM images of control experiments using the same particles but without the anti-EGF antibody. (g) Box plot of the number of 5 nm NP labels detected on NP-EGF (left) and control (right). The TEM data confirm a selective binding of 5 nm NP labels to NP-EGF. (h) EGFR phosphorylation in HeLa after treatment with NP-EGF and pegylated NPs for 2h and 8h. All phosphorylation levels are normalized to the reference condition: 15min of incubation with 1.6nM free EGF (last column). The measured phosphorylation levels confirm EGFR activation through nanoconjugated EGF. (i) UV-Vis spectra of NP-PEG (red) and NP-EGF before (yellow) and after (blue) incubation with A431 cells in serum containing medium for 4h. The superposition of the graphs confirms the stability of NP-EGF against agglomeration.