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Placental insulin signaling is not affected by maternal obesity. Western blots of phosphorylation (P) and/or expression of AKT, ERK1/2, GRB2, IRS1, and PI3K p85 in placental homogenates from control and obese dams (A). After normalization to total protein stain (Amido Black), the mean density of control samples was assigned a value of 1. The summarized Western blot data are shown in histogram (B). Data are presented as mean + SEM. Control, n = 5; Obese, n = 7.
