. 2014 Mar;53(2):198–203.

Table 1.

Zebrafish response (seconds; mean ± 1 SD; n= 10 per group) to anesthetic agents

Agent	Dose	Loss of equilibrium	Slowing of opercular movement	Lack of response to tail-fin pinch	Recovery time
MS-222	150 mg/L	111 ± 92	513 ± 99	252 ± 89	140 ± 51
Lidocaine hydrochloride
	300 mg/L	196 ± 95	323 ± 234	not applicable	206 ± 148
	325 mg/L	365 ± 137^a	90 ± 190^a	51 ± 162^a	346 ± 310^a
	350 mg/L	34 ± 20^a	78 ± 30^a	101 ± 18^a	116 ± 104
Metomidate hydrochloride
	2 mg/L	80 ± 136	100 ± 211^a	not applicable	169 ± 14
	4 mg/L	159 ± 53	186 ± 242^a	not applicable	313 ± 207^a
	6 mg/L	113 ± 45	260 ± 55^a	not applicable	625 ± 247^a
	8 mg/L	40 ± 18^a	166 ± 47^a	not applicable	337 ± 150^a
	10 mg/L	37 ± 19^a	136 ± 25^a	not applicable	548 ± 253^a
Isoflurane	0.5 mL/L	not applicable	395 ± 136	not applicable	253 ± 166
Gradual cooling	—	313 ± 133^a	313 ± 133^a	313 ± 133	0 ± 0*

An entry of ‘not applicable’ indicates that the reflex was not lost.

Value significantly (P < 0.05) different from that for MS222 after pairwise analysis with the Mann–Whitney–Wilcoxon test and Bonferroni correction.