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. 2014 Apr;62(4):237–250. doi: 10.1369/0022155413519650

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© The Author(s) 2014

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 5. — NOD mice on neutral drinking water (NOD-N) have decreased production of IL17 by CD4⁺ T cells as compared with mice on acidic water (NOD-A). (A) The percentage of CD4⁺ splenocytes and lymphocytes from the small intestine (SI) and colonic (Co) lamina propria (LP) of 2-week-old (left panel) and 8–10-week-old (right panel) NOD-A (open squares) and NOD-N (closed circles) mice. (B-C) Percentages of CD4⁺ lymphocytes that were also (B) IL17 or (C) IFNγ⁺. (D) Comparison of the ratio of CD4⁺IL17⁺ and CD4⁺IFNγ⁺ cells in 2-week-old (left panel) and 8–10-week-old (right panel) NOD-A and NOD-N mice. At 2 weeks: NOD-A, n=5; NOD-N, n=6. At 8–10 weeks: NOD-A, n=4; NOD-N, n=4). Significance was determined using Welches’ t-test at p<0.5. Absolute numbers of cells in each population can be seen in Figure S2.