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Calculated folding free energy changes compared with the experimental data from ProTherm database for five types of mutations. The corresponding optimal dielectric constants are provided as well. (a) Mutation involving Asp residue; optimal dielectric constants ε1 =20 and ε2 =5 (b) Glu; ε1 =18 and ε2 =10 (c) Arg; ε1 =28 and ε2 =6 (d) His; ε1 =18 and ε2 =4 (e) Lys; ε1 =25 and ε2 =5.
