Table 3.
Primer sequences and product sizes used in this study.
| Pathogen | Target | Sequence 5′-3′ | Size | References |
|---|---|---|---|---|
| Shigella and EIEC | ipaH | AGGTGACACTATAGAATA aACCATGCTCGCAGAGAAACT | 213 | [10] |
| GTACGACTCACTATAGGGA bTCAGTACAGCATGCCATGGT | ||||
|
| ||||
| Vibrio parahaemolyticus | tlh | AGGTGACACTATAGAATAACTCAACACAAGAAGAGATCGACAA | 246 | [30] |
| GTACGACTCACTATAGGGAGATGAGCGGTTGATGTCCAA | ||||
|
| ||||
| EHEC and EPEC | eaeA | AGGTGACACTATAGAATAAGGTCGTCGTGTCTGCTA | 293 | [31] |
| GTACGACTCACTATAGGGACCGTGGTTGCTTGCGTTTG | ||||
|
| ||||
| Salmonella | invA | AGGTGACACTATAGAATAGTGAAATTATCGCCACGTTCGGGCAA | 323 | [32] |
| GTACGACTCACTATAGGGATCATCGCACCGTCAAAGGAACC | ||||
|
| ||||
| Yersinia | ail | AGGTGACACTATAGAATATAATGTGTACGCTGCGAG | 389 | [5] |
| GTACGACTCACTATAGGGAGACGTCTTACTTGCACTG | ||||
|
| ||||
| Vibrio cholerae | ctx | AGGTGACACTATAGAATAACAGTAACTTAGATATTGCTCCAG | 507 | This study |
| GTACGACTCACTATAGGGAACCATTCTTAAAAGTAATGATAGCCA | ||||
|
| ||||
| Campylobacter jejuni | mapA | AGGTGACACTATAGAATACTATTTTATTTTTGAGTGCTTGTG | 627 | [33] |
| GTACGACTCACTATAGGGAGCTTTATTTGCCATTTGTTTTATTA | ||||
|
| ||||
| Noroviruses GI | RDRP | AGGTGACACTATAGAATACGCTGGATGCGCTTCCATGA | 162 | [21] |
| GTACGACTCACTATAGGGAGCAAGAGGGTCAGAAGCATT | ||||
|
| ||||
| Rotavirus | VP6 | AGGTGACACTATAGAATAAAGTCTTCCACATGGAGGT | 210 | Modified [21] |
| GTACGACTCACTATAGGGAARRTTICCAATTCCTCCAGT | ||||
|
| ||||
| Norovirus GII | RDRP | AGGTGACACTATAGAATACAGACAAGAGCCAATGTTCA | 279 | Modified [21] |
| GTACGACTCACTATAGGGATTTCTAATCCAGGGGTCAATT | ||||
|
| ||||
| Human astrovirus | ORF1a | AGGTGACACTATAGAATACGTCATTATTTGTTGTCATA | 326 | [21] |
| GTACGACTCACTATAGGGACATGTGCTGCTGTTACTATG | ||||
|
| ||||
| Enteric adenovirus | Hexon | AGGTGACACTATAGAATATGTACAAGCCAGNTGTAGCTC | 388 | This study |
| GTACGACTCACTATAGGGAAAGCAGTAATTTGGCANTTCGT | ||||
|
| ||||
| Human bocavirus 1c | VP1 | AGGTGACACTATAGAATAAAACCCATCACTCTCAATGCTT | 412 | This study |
| GTACGACTCACTATAGGGACAGTATGTCTTCTTTCTGGACG | ||||
|
| ||||
| Human bocavirus 2 | VP1 | AGGTGACACTATAGAATAAAATCCACCACTATCCATGCTC | 412 | This study |
| GTACGACTCACTATAGGGACGGTGTGTCTTCTTTCTGGTCT | ||||
aUniversal primers-F: AGGTGACACTATAGAATA; buniversal primers-R: GTACGACTCACTATAGGGA; cthe primers for HBoV1 and HBoV2 are equally mixed, the amplicon sizes of both PCR products are exactly the same, and the primers are located at the same position in the corresponding viral genome; this method is able to identify the presence of HBoV1 and HBoV2 but cannot classify the subtypes.