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Chromatogram of hydroalcoholic extract of the leaves of T. catappa dissolved in MeOH : H2O (1 : 1 v/v), at 10 mg/mL. The method utilized a hydro column at a flow rate of 1 mL/min with a gradient of 5–60% MeOH for 60 min HPLC-PDA (Jasco), 270 nm. The UV spectra from t r 14.75 (peak 1) min and at t r 18.86 min (peak 2), characteristic of the α- and β-anomers of punicalagin (1, 2) and ellagic acid (3).
