Table 1. Primers used for H5 and H9 subtyping of avian influenza viruses from Egypt, 2010–2012* .
| Primer | Sequence, 5′→3′ | Reference |
|---|---|---|
| M30F2/08 | ATGAGYCTTYTAACCGAGGTCGAAACG | (12) |
| M264R3/08 | TGGACAAANCGTCTACGCTGCAG | |
| H5–155f | ACACATGCYCARGACATACT | (13) |
| H5–699r | CTYTGRTTYAGTGTTGATGT | |
| H9–151f | CTYCACACAGARCACAATGG | |
| H9–638r | GTCACACTTGTTGTTGTRTC | |
| BDH9–4F2 | CAAGCGTGACAACAGAAAATTTGG | Designed in-house† |
| BDH9–2R2 | CTCCTGAGAGAACGTGTCCATACC | |
| H9PROB | FAM CTTACTCGCAATGTCTGGCCTGGTTTTAG BHQ1 | |
| AH5b_Forward | GGA ATGYCCCAAATATGTGAAATCAA | (14) |
| AH5b_R | CCACTCCCCTGCTCRTTGCT | |
| H5PROB | FAM TACCCATACCAACCATCTACCATTCCC BHQ1 | |
| Inf-A F | ACCRATCCTGTCACCTCTGAC | |
| Inf-A R | AGGGCATTYTGGACAAAKCGTCTA | |
| Inf-A POB | FAM TGCAGTCCTCGCTCACTGGGCACG BHQ1 |
*Typing by reverse transcription PCR and quantitative reverse transcription PCR. †St. Jude Children’s Research Hospital, Memphis, TN, USA.