Figure 5.

COMT hyperactivity does not alter the level of TH or DAT in the striatum. (A) Striatum (STR) extracts from COMT-OE and control mice probed with anti-TH antibody shows no difference in the level of TH protein. Cerebellum (CER) extracts were used as a negative control for the TH and DAT antibodies. An anti-tubulin antibody was used as a loading control and to normalize band density. (B) No difference in the level of DAT protein was detected between genotypes. (C) Quantification of the TH bands. (D) Quantification of the DAT bands. Control n = 3 mice, COMT-OE n = 3 mice.