Fig. 1.

SEMA7A gene expression in murine mammary cells. a Total RNA was purified from benign EpH4 mammary cells and E0771 and DA-3 mammary tumor cells and analyzed using qRT-PCR primers (SABiosciences) for the expression of murine semaphorin7A. b Calcein AM-labeled DA-3 cells (0.1 × 10⁶) were placed in the upper chamber of BD Fluoroblok 0.8-μm transwell inserts in serum-free DMEM with and without rmSEMA7A (R&D Systems) and incubated for 12 h using 10 % FBS-DMEM as a positive control. The fluorescent intensity of migrated DA-3 was measured at 12 h and compared to serum-free DMEM as percentage increase. Data are representative of three independent experiments. Statistical comparisons of paired groups were determined by Student’s t tests. * <0.05, ** <0.01