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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Chem Biol. 2014 Feb 20;21(3):389–397. doi: 10.1016/j.chembiol.2014.01.005

Fig. 2.

Fig. 2

Disk diffusion assays for PTM and PTN susceptibility of recombinant Streptomyces albus J1074 strains that carry varying expression constructs. (A) PTM and PTN susceptibility: J1074 (wild-type), SB12009 (J1074/pBS12016 and ptmP1 expressing), SB12010 (J1074/pBS12017 and ptmP2 expressing), SB12011 (J1074/pBS12018 and ptmP3 expressing), and SB12012 (J1074/pBS12019 and ptmP4 expressing), challenged with 5, 10, 15, and 20 μg of PTM or 1.25, 2.5, 5, and 10 μg of PTN (clockwise from top filter, respectively). (B) PTN susceptibility: SB12011 (J1074/pBS12018 and ptmP3 expressing); SB12015 [J1074/pBS12026 and ptmP3 (C162A) expressing], SB12016 [J1074/pBS12027 and ptmP3 (C162Q) expressing], and SB12017 (J1074/pBS12028 and ptmP3 (C162L) expressing], challenged with 1.25, 2.5, 5, and 10 μg of PTN (clockwise from top filter, respectively). See also Figure S2 and Table S1.