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. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Oncogene. 2013 Jun 10;32(49):5551–5562. doi: 10.1038/onc.2013.207

Figure 3. A MCF-7 Cancer Cell Line Stably Overexpressing Recombinant Human PLD2 shows enhanced cancer cell invasion.

Figure 3

A, Simplified scheme of MIEG-PLD2 mRNA. B–D, Immunofluorescence of GFP in puromycin-resistant, stable MCF-7·pMIEG cells overexpressing either GFP vector, PLD1 or PLD2. E, Western-blot analyses of cell lysates. F, Effect of PLD overexpression on cell proliferation. G, Effect of PLD overexpression on PLD activity. H-I, Effect of PLD overexpression on physiological functions (cell invasion and chemotaxis, respectively). Triplicate results are mean ± SEM. * denotes statistically significant (p<0.05) increases respect to controls.