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. Author manuscript; available in PMC: 2014 Jun 5.
Published in final edited form as: Oncogene. 2013 Jun 10;32(49):5551–5562. doi: 10.1038/onc.2013.207

Figure 7. The mechanism that regulates PLD-mediated cell invasion and metastasis involves PA, Rac2 and Grb2.

Figure 7

A, PA sensor used for transfection into MDA-MB-231 or MCF-7 cells. B, Immunofluorescence of PA sensor expression. White arrowheads denote PA sensor localized to a membranous surface that contained PA. C, Cell invasion of MCF-7 cells stably expressing lipase-inactive constructs (PLD1-K866R or PLD2-K758R). D, Tumor size of mice injected with MCF-7 cells stably expressing PLD2, a WASp construct or a combination of the two. E, EGF-mediated cell invasion in cells that overexpress PLD2, Grb2 and Rac2 constructs (WT and SH2-binding deficient). F, PLD2, Grb2 and Rac2 protein expression in MDA-MB-231 cells. G, EGF-mediated cell invasion in cells silenced with siRNA for Grb2 and/or PLD2 or with siRNA for Rac2.