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Structural investigation of MPO by CD. CD near-UV visible spectra of 0.5 mg/ml free MPO (red), 0.5 mg/ml LDL (mauve), HOCl-LDL (oxidant/lipoprotein molar ratio = 50:1, orange), or 0.5 mg/ml MPO incubated with 0.5 mg/ml LDL (light green) or with 0.5 mg/ml HOCl-LDL (oxidant/lipoprotein molar ratio = 50:1, dark green) in 5 mM PBS (pH 7.4) at 25°C. For details, see the Experimental Section. mdeg, millidegree.
