Fig. (2).

HDAC2 and 5-mC immunofluorescence, and DAPI labeling. Representative high magnification images of a nucleus of a cell in a 12-month-old mouse showing DAPI labeling (A and E) and fluorescence labeling of 5-methylcytidine (5-mC) (red) in B and F, and HDAC2 (green) in C and G, at the corresponding location and focal plane, with merged pictures in D and H. All images represent one stack taken with the SI-SD system (see text for more details). Note the white arrow indicates the colocalization between HDAC2 and 5-mC (yellow). Images E–H show a three-dimensional reconstruction of a representative image stack. The crossing lines indicate the position within the X–Y view at which the Y–Z (E1–H1) and X–Z (E2–H2) views were generated. Scale bar = 10 µm.