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. Author manuscript; available in PMC: 2014 Mar 26.
Published in final edited form as: J Biol Chem. 2006 Dec 15;282(9):6609–6618. doi: 10.1074/jbc.M609828200

FIGURE 3. ITC measurements with wild-type MeTr and N199A variant.

FIGURE 3

The experiments were performed by titrating 50μm MeTr (as monomer) in 0.1 m buffer, 0.1 m NaCl, 1 mm DTT with 1.5 mm 6S-CH3-H4folate in the same buffer. For wild-type and N199A MeTr, the buffers were MES, pH 5.1, and Tris-HCl, pH 7.6. A, for the N199A variant (measured at pH 5.1), the fit parameters are n = 1.2, K = 1.26 (± 0.04) × 106 m, ΔH = –13200 ± 40 cal/mol, ΔS = –17.1 cal/mol-K. B, for wild-type MeTr (measured at pH 7.6), the fit parameters are n = 1.5, K = 3.01 (± 0.13) × 104 m, ΔH = –8187 ± 209 cal/mol, ΔS = –7.44 cal/mol-K.