Table 1.
Primary and secondary antibodies used in the immunohistofluorescence studies
| Antibody | Dilution source |
|---|---|
| Primary | |
| Rabbit anti-nNOS polyclonal antiserum (160870) | 1:500 Cayman Chemical (Ann Arbor, MI, USA) |
| Rabbit anti-vimentin polyclonal antiserum (ab8545) | 1:500 Abcam (Cambridge, UK) |
| Goat anti-vesicular ACh transporter polyclonal antiserum (ab34852) | 1:200 Abcam |
| Sheep anti-TH polyclonal antiserum (ab113) | 1:400 Abcam |
| Sheep anti-cGMP polyclonal antiserum | 1:2000a |
| Secondary | |
| Alexa 594 conjugated donkey anti-rabbit (A21207) | 1:400 Molecular Probes |
| Alexa 488 conjugated donkey anti-sheep (A11015) | 1:400 Molecular Probes |
| Alexa 488 conjugated donkey anti-goat (A11055) | 1:400 Molecular Probes |
a
A generous gift from Professor De Vente, Maastricht University, Holland. Manufacturer catalogue numbers are between brackets.