Table 3.

EF₅₀ and E_max values obtained from the frequency–response contraction curves of SAMR1 and SAMP8 detrusor muscle under different conditions

	SAM-R1			SAM-P8
♀	EF50	Emax (% maximum)	n	EF50	Emax (% maximum)	n
	Control	18.3 ± 2.15		94.6 ± 1.45 (45 Hz)	12		9.7 ± 0.72***	97.1 ± 1.22 (25–30 Hz)	14
Atropine (1 μM)	16.1 ± 1.28	53.4 ± 4.73###,†††	11	7.0 ± 0.47***	56.2 ± 7.21†††	9
Atropine + αβ-MeATP (100 μM)	18.8 ± 2.95	21.2 ± 2.24ΦΦ	10	6.64 ± 0.95**	28.3 ± 3.24ΦΦΦ	9
Atropine + αβ-MeATP + Indomethacin (30 μM)	13.6 ± 1.51	15.8 ± 5.66	6	12.5 ± 0.61	11.3 ± 3.73Δ	6

	SAM-R1			SAM-P8
♂	EF50	Emax (40 Hz)	n	EF50	Emax (20–25 Hz)	n
	Control	16.5 ± 1.34		94.4 ± 1.56 (40 Hz)	13		7.53 ± 0.86***	97.6 ± 1.77 (20–25 Hz)	10
Atropine (1 μM)	12.9 ± 0.86	27.8 ± 1.98††	7	6.04 ± 0.53***	49.2 ± 8.72*†††	7
Atropine + αβ-MeATP (100 μM)	13.1 ± 1.87	15.2 ± 1.77Φ	6	9.02 ± 1.28	17.1 ± 4.25ΦΦ	6
Atropine + αβ-MeATP + Indomethacin (30 μM)	13.2 ± 1.59	10.0 ± 1.23	6	15.3 ± 5.04	11.5 ± 2.34	6

E_max (maximum response) values are expressed relative to the maximum response in each frequency–response curve and the EF₅₀ represents the negative logarithm of the frequency that produces 50% of that maximum. The frequency at which the maximum was obtained is shown in brackets. The EF₅₀ and E_max are expressed as the mean ± SE:

^††P < 0.01,

^†††P < 0.001 versus the control group;

^ΦP < 0.05,

^ΦΦP < 0.01,

^ΦΦΦP < 0.001 versus the atropine-treated group;

^ΔP < 0.05 versus the atropine + αβ-MeATP-treated group (paired t-test, comparisons made in absolute values);

^*P < 0.05,

^**P < 0.01,

^***P < 0.001 versus SAMR1;

^###P < 0.001 versus male (anova followed by unpaired t-test, comparisons made in normalized values).