Figure 2.

SHP-2 inhibitor NSC-87877 modulates the expression of associated factors in the spinal cord. (A and B) EAE was induced in C57BL/6 mice and NSC-87877 was administered to mice as in Figure 1A. (A) Numbers of spleen cells from vehicle-and NSC-87877-treated mice after immunization are shown. (B) Relative proportions of spinal cord infiltrating CD4⁺ and CD8⁺ T lymphocytes from vehicle-and NSC-87877-treated mice on day 16 post immunization are displayed. (C) The expressions of inflammatory factors in the spinal cord from vehicle-and NSC-87877-treated mice on day 16 post immunization were measured via quantitative real-time RT-PCR. (D) RT-PCR analysis of ICAM-1, VCAM-1, LFA-1 and VLA-4 mRNA expression in the spinal cord from vehicle-and NSC-87877-treated mice on day 16 post immunization. (E) NSC-87877 was administered to mice as in Figure 1A, sodium fluorescein was injected i.v. on day 16 post immunization, and the spinal cord was dissected 30 min later and evaluated for fluorescein incorporation. Data are expressed as mg sodium fluorescein mg⁻¹ of spinal cord protein. (F) The mRNA levels of occludin, ZO-1, claudin 3, and claudin 5 in the spinal cord from vehicle-and NSC-87877-treated mice on day 16 post immunization were measured by RT-PCR. The housekeeping gene β-actin mRNA was used to normalize the relative amounts of mRNA. Values are given as the mean ± SEM. *P < 0.05; **P < 0.01 versus EAE.