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. 2014 Mar 18;171(7):1706–1721. doi: 10.1111/bph.12565

Figure 4.

Figure 4

Blocking the infiltration of CD8+ T-cells into the CNS is responsible for the amelioration of EAE by NSC-87877. (A) Cells harvested from draining lymph nodes of vehicle-or NSC-87877-treated mice on day 10 (before onset of disease) or day 16 (peak of disease) post immunization were stained for CD4, CD8 and B220 followed by flow cytometric analysis. Cell numbers were calculated and displayed as percentages. **P < 0.01. (B, C) Ag-specific donor CD8+ T-cells were obtained from draining lymph nodes of vehicle-or NSC-87877-treated mice on day 10 post immunization. Then the cells were cultured in the presence of MOG35–55 for 72 h. Cultured cells were then adoptively transferred into normal C57BL/6 recipients (5 × 107 per mouse), and the EAE severity (D), weight change (E) and incidence (F) were observed daily. Values are given as the mean ± SEM of six mice per group.