Figure 8.

CSY0073 fails to decrease IL-8 production or NF-κB activation in lung respiratory epithelial cells. (A) MucilAir™ primary air-liquid lung epithelial cells were pretreated for 24 h at the basal side with vehicle (V: citric acid) 20 μM of CSY0073 (CSY) or AZM then stimulated at the apical side with 50 μL of Pseudomonas aeruginosa LPS 10 μg·mL⁻¹ for 24 h. Data are the mean ± SEM of one experiment performed in quadriplicate (four different patients). Statistical analysis was with Student's t-test. (B) BEAS-2B cells were treated for 24 h with V, CSY0073 (CSY) or AZM. Treatment with these drugs was started 24 h before or concomitantly with the 24 h P. aeruginosa LPS 1 μg·mL⁻¹ treatment. Data are the mean ± SEM of three experiments performed in triplicate. (C) and (D). (C) A549/NF-κB-luc cells were pretreated for 24 h with V, 20 μM of CSY0073 (CSY) or AZM then exposed for 6 h to 1 μg·mL⁻¹ P. aeruginosa LPS, 10 nM TNF-α, or 0.1 nM IL-1β. IL-8 was measured in the supernatants and (D) NF-κB luciferase activity in the cell lysates. Data are the mean ± SEM of five experiments performed in triplicate. Statistical analysis was done with anova followed by Bonferroni's multiple comparisons test (B, C, and D). Each value was compared with the positive control condition (V for all three stimuli). *P < 0.05 and ****P < 0.0001.