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. 2014 Mar 26;9(3):e92188. doi: 10.1371/journal.pone.0092188

Figure 2. Vector dose dependence of amiR-155-PLBr, shPLBr and PLB mRNA expression in cardiomyocytes (CM) at post transduction days 4 and 14.

Figure 2

CM were transduced with indicated doses of scAAV6-shPLBr and scAAV6-amiR155-PLBr and analyzed for expression of processed shPLBr and amiR155-PLBr normalized to 18 S rRNA (A) as well as PLB mRNA normalized to GAPDH mRNA using qRT-PCR (B). All values in A and B were normalized to that obtained for the cells transduced with the lowest scAAV6-shPLBr vector dose. The column labeled no add contains nontransduced cells. *p<0.05 vs. cells transduced with the same dose of the scAAV6-shPLBr vector. #p<0.05 vs. non-transduced cells. a.u., arbitrary units.