Table 3. Additional aspartate leads to a production of bigger cells and more C-period cells.

Strain	genotype	a.a.	B(%)	C(%)	D(%)	Cell size(μm)	Doubling time(min)
BW25113	Wild type	–	39.6	39.7	20.6	2.09±0.14	116.6±0.2
		Asp	29.0	47.2	23.8	2.29±0.13	87.3±1.2
		Gly	34.7	44.0	21.3	2.04±0.13	99.7±0.3
		Tyr	38.4	41.4	20.2	2.04±0.16	114.5±0.8
MOR814	ΔaspC	–	46.0	34.9	19.1	1.80±0.14	129.6±0.8
		Asp	30.5	45.0	24.5	2.16±0.15	98.3±0.5
		Gly	45.5	36.4	18.1	1.86±0.22	121.2±1.7
		Tyr	68.8	19.3	11.9	1.76±0.12	430.9±3.5

Exponentially growing cells in ABT medium supplemented with amino acids as indicated were fixed in 70% ethanoland then analyzed by flow cytometry as described in Materials and Methods. The number of cells in B-, C- and D-period was calculated using a software provided by BD and cell size was determined by fluorescent microscopy. Each calculation includes more than 100 cells. The values are average of three individual experiments, standard errors are given.