Table 3. Additional aspartate leads to a production of bigger cells and more C-period cells.
Strain | genotype | a.a. | B(%) | C(%) | D(%) | Cell size(μm) | Doubling time(min) |
BW25113 | Wild type | – | 39.6 | 39.7 | 20.6 | 2.09±0.14 | 116.6±0.2 |
Asp | 29.0 | 47.2 | 23.8 | 2.29±0.13 | 87.3±1.2 | ||
Gly | 34.7 | 44.0 | 21.3 | 2.04±0.13 | 99.7±0.3 | ||
Tyr | 38.4 | 41.4 | 20.2 | 2.04±0.16 | 114.5±0.8 | ||
MOR814 | ΔaspC | – | 46.0 | 34.9 | 19.1 | 1.80±0.14 | 129.6±0.8 |
Asp | 30.5 | 45.0 | 24.5 | 2.16±0.15 | 98.3±0.5 | ||
Gly | 45.5 | 36.4 | 18.1 | 1.86±0.22 | 121.2±1.7 | ||
Tyr | 68.8 | 19.3 | 11.9 | 1.76±0.12 | 430.9±3.5 |
Exponentially growing cells in ABT medium supplemented with amino acids as indicated were fixed in 70% ethanoland then analyzed by flow cytometry as described in Materials and Methods. The number of cells in B-, C- and D-period was calculated using a software provided by BD and cell size was determined by fluorescent microscopy. Each calculation includes more than 100 cells. The values are average of three individual experiments, standard errors are given.