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. 2014 Mar 26;9(3):e92594. doi: 10.1371/journal.pone.0092594

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© 2014 Monteiro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) H⁺ flux during regeneration after vivo-MO mediated atp6v1e1b knockdown, 2 h after proximal (A) and distal (B) amputation. *statistical significant results (p<0.05). (C, D) Caudal fin regeneration after atp6v1e1b vivo-MO delivery to atp6v1e1b^hi577aTg/+ (AB) fish, 2 h after proximal amputation (C) and 16 h after distal amputation (D). (C’, D’) Percentage of regenerate area in atp6v1e1b knocked down regions compared to the control vivo-MO (cVivo-MO). Vivo-MOs were delivered 2 h (C’) or 16 h (D’) after both proximal and distal amputation. hpa: hours post amputation. For each panel, n = 8.