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. 2014 Mar 26;9(3):e92703. doi: 10.1371/journal.pone.0092703

Figure 5. Flow cytometric measurements of wild-type and mutant LDLR internalization activity in transfected HEK-293 cells.

Figure 5

The transfected cells were incubated in serum-free media containing 20 μg/ml Dil-LDL at 37°C for 4 hours. The upper-right area of the dot plots represents EGFP and LDLR double positive cells. (A) Transfected with wild-type; (B) Transfected with the G615V mutant LDLR; (C) Transfected with the C201F mutant LDLR; (D) The histogram shows the percentage of fluorescence for each of the mutations relative to wild-type LDLR. The results are representative of the means ± SD for three independent experiments.