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. 2014 Mar 26;9(3):e92719. doi: 10.1371/journal.pone.0092719

Figure 6. Transfection of NIH/3T3 with EV71:BS viral RNA induces productive infection.

Figure 6

Overnight seeded NIH/3T3 and Vero cells were inoculated with virus supernatant harvested from NIH/3T3 cells previously transfected viral RNA extracted from EV71:BS, EV71:TLLm, and EV71:TLLmv. (A) Cells were imaged using inverted light microscope at 24 hpi to observe induced CPE. (B) Cells were harvested at 7 dpi, coated onto Teflon slides, probed with pan-enterovirus antibody, and stained with anti-mouse FITC-conjugated antibody.