Figure 8. Cooperativity correlation difference plots highlight differences in pairwise mechanical couplings between the wild type and each mutant.

Red indicates increased correlated flexibility within the mutant structure, whereas blue indicates increased correlated rigidity. White indicates no change. Notice in most mutants (i.e., triple mutant), changes in cooperativity correlation occur throughout the Fv structure, whereas they are primarily isolated to the VH domain in the quadruple mutant.