Figure 6. G_βγ plays a central role in HCA₁-induced ERK1/2 activation.

Serum-starved CHO-HCA₁ cells (a and b) or L6 cells (c) were pretreated with DMSO or 20 μM M119K for 4 hrs, and the cells were then stimulated with 10 mM L-Lactate (a) or 300 μM 3,5-DHBA (b) for CHO-HCA₁ cells and 3 mM 3,5-DHBA for L6 cells (c) for indicated time periods. The data shown are representative of at least three independent experiments. Error bars, S.E. for three replicates. IB, immunoblot; P-ERK, phospho-ERK.