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. 2014 Mar 26;9(3):e93141. doi: 10.1371/journal.pone.0093141

Figure 4. Synthesis of RpoS by wild type but not bbd18-expressing B. burgdorferi .

Figure 4

Immunoblot analysis of cell lysates from strains B31-S9 (wt), B31-S9ΔrpoS (ΔrpoS) and B31-S9/pBSV2*-flaBp-bbd18 (wt/flaBp-bbd18) grown under rpoS-inducing conditions. Cell lysates were analyzed using RpoS antiserum (A) or a mouse monoclonal antibody to flagellin (B) to assess flagellin levels as a protein loading control. Cell lysates and immunoblot for flagellin were the same as those used in Fig. 1. Positions of molecular mass standards are shown on the left in kiloDaltons (kDa).