Figure 4. CFTR inhibition by CFTR_inh-172 affects lipoxin A4, inflammatory parameters, and E. coli number in BAL.

Mice were pretreated with either vehicle or CFTR_inh-172 and then intratracheally challenged with E. coli (10⁷ cfu). At 4 h, mice were killed to collect BAL and measure the relevant parameters. A. BAL lipoxin A4/MIP2 ratio. B. BAL lipoxin A4/neutrophils ratio. C. BAL MIP-2/neutrophils ratio. D. BAL protein levels. E. BAL E. coli numbers. F. Cytological changes of BAL proinflammatory cells and E. coli infection. Black arrows indicate infected cells; Red arrows denote E. coli. Wright’s staining, magnification×100. Values are presented as mean ± SD. Sample size and P values are indicated in each panel.