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. 2014 Mar 26;9(3):e93003. doi: 10.1371/journal.pone.0093003

Figure 6. Deficiency of CFTR affects plasma PAF levels and PAF-AH activity in LPS-induced lung inflammation (A–B).

Figure 6

Wildtype and F508del mice were intratracheally challenged with LPS (5 mg/kg) and killed at 24 h to collect plasma. A. Plasma PAF levels were measured by HPLC-MS. B. Plasma PAF-AH activity (In control group, PBS was added into reaction system; naïve wildtype and F508del groups, plasma was collected from PBS-treated mice. Repeated measures two-way ANOVA was used for statistical analysis. *P<0.05, **P<0.01, and ***P<0.001 for F508del + LPS group versus wildtype + LPS group. Inhibition of PAF-AH by MAFP worsens acute lung inflammation in F508del mice (C–E). F508del mice were divided into two groups to receive either vehicle or MAFP (1 mg/kg) treatment 20 min before an intratracheal challenge of LPS (5 mg/kg). The mice were killed at 24 h to collect blood and BAL. C. PAF-AH activity. Repeated measures two-way ANOVA was used for statistical analysis. *P<0.05, **P<0.01, and ***P<0.001 for F508del mice receiving MAFP versus F508del mice receiving vehicle. Both groups were intratracheally challenged with LPS. D. BAL protein levels. E. BAL neutrophils. Values are presented as mean ± SD. Sample size and P values are indicated in each panel.