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. 2014 Mar 26;9(3):e93058. doi: 10.1371/journal.pone.0093058

Figure 2. Probenecid inhibits ATP-induced dye uptake in HEK-hP2X7 cells.

Figure 2

(A) Ethidium+ uptake was induced in HEK-hP2X7 expressing cells by the addition of 1 mM ATP (as denoted by the arrow) in low divalent physiological solution. Control ethidium uptake to ATP is shown in black squares and ethidium uptake in the presence of 2.5 mM probenecid (pre-incubated for 10 minutes at 37°C) is shown in open circles. (B) Concentration response curves for probenecid on human P2X7 dye uptake responses induced by 0.2 mM or 1 mM ATP. Data was calculated at 300 second timepoint as baseline corrected endpoint data and normalised to the ATP control. Data was fit using a normalised response variable slope in GraphPad Prism. (C) Concentration response curves for agonist ATP in the presence of increasing concentrations of probenecid (0.5 mM, 1 mM and 5 mM) showing a rightward shift.