Skip to main content
. 2014 Mar 26;9(3):e93463. doi: 10.1371/journal.pone.0093463

Figure 2. Binding of toxin panel to Gb3 as measured by ELISA.

Figure 2

All four toxins bound Gb3; however, Stx1a and 211 bound Gb3 with a significantly higher affinity than Stx2a or 122 (p<0.01). Additionally, when toxins with identical B subunits were compared, Stx1a vs 211 and Stx2a vs 122, there was no difference in binding (p>0.05). Error bars represent standard error of the mean (SEM); n = four biological replicates. Toxin-Gb3 binding was compared by Two-Way ANOVA with Tukey's adjustment for multiple comparisons.