Figure 3. Stx translocation through a polarized HCT-8 cell monolayer after 20 ng was applied to the apical chamber.

The Stx1a B subunit was associated with statistically greater toxin translocation compared to the Stx2a B subunit. A greater amount of Stx1a and 211 was recovered from the basolateral chamber as compared to Stx2a and 122 at 2.5 and 24(p<0.05). Error bars represent SEM. n = 4 biological replicates