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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Hepatology. 2014 Feb 25;59(4):1381–1392. doi: 10.1002/hep.26912

Figure 2. Chronic alcohol exposure markedly increases hepatic VLDLR expression.

Figure 2

Male C57BL/6 mice weighing 25 ± 0.5 g (means ± SD) were pair-fed liquid diets with or without ethanol for 5 weeks. Total RNAs were isolated from liver and adipose tissues. (A & B) Real time RT-PCR analysis of genes involved in hepatic lipoproteins delivery (A) and metabolism (B). (C) Real time RT-PCR analysis of VLDLR gene expression (relative to epididymal fat) in epididymal fat and the liver, as well as VLDLR and LPL gene expressions (relative to PF) in epididymal fat pad. All values are denoted as means ± SD Data (n = 8). * p < 0.05 when compared with corresponding PF. Both whole liver tissue and plasma membrane proteins were isolated and subjected to Western blot for the detection of VLDLR protein abundance (D & E). All values are denoted as means ± SD Data (n = 8). * p < 0.05 when compared with corresponding PF. PF, pair-fed; AF, alcohol-fed.