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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Leukemia. 2013 Sep 26;28(4):917–927. doi: 10.1038/leu.2013.279

Figure 1. Generation of a CS1-specific CAR and detecting its expression in CAR-transduced NK cells.

Figure 1

A) Schematic representation of the CS1-CAR lentiviral construct that we generated. B) Western blotting analysis of CS1-CAR expression using a CD3 ζ-specific Ab. Data shown are representative of three experiments with similar results. C) Expression of chimeric CS1 scFv on the surface of FACS-sorted NK-92 and NKL cells transduced with the CS1-CAR construct (NK-92-CS1-CAR and NKL-CS1-CAR) was analyzed by flow cytometry after cells were stained with an anti-myc antibody or IgG1 isotype control. Data shown are representative of three experiments with similar results.