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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Leukemia. 2013 Sep 26;28(4):917–927. doi: 10.1038/leu.2013.279

Figure 4. Enhanced target recognition of NK-92-CS1-CAR cells depends on expression of CS1 on MM cells.

Figure 4

A) Flow cytometric staining for CS1 protein or IgG isotype control (dotted line) on the surface of U266 cells overexpressing CS1 (U266-CS1, solid heavy line) or an empty vector control (U266-Vector, solid light line). B) Cytotoxicity of mock- or CS1-CAR-transduced NK-92 cells (NK-92-EV and NK-92-CS1-CAR, respectively) against U266-Vector and U266-CS1 cells. U266-Vector or U266-CS1 cells were incubated with NK-92-CS1-CAR or NK-92-EV cells at different Effecor/Target (E/T) ratios for 4 h. Specific lysis was determined using a standard chromium-51 release assay. * indicates P < 0.05. C) NK-92-CS1-CAR or NK-92-EV cells were co-cultured with an equal number of U266-Vector or U266-CS1 myeloma cells for 24 h. Supernatants were then harvested for measurement of IFN-γ secretion using ELISA.