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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Leukemia. 2013 Sep 26;28(4):917–927. doi: 10.1038/leu.2013.279

Figure 6. CS1-CAR-transduced NK-92 cells enhance killing of primary human myeloma cells.

Figure 6

A) Flow cytometric staining for CS1 protein or IgG isotype control, demonstrating that CD138+ primary myeloma cells highly express CS1. The open and filled histograms represent staining with isotype-matched control antibodies and anti-CS1 antibodies, respectively. Data shown are representative of 2 out of 6 patient samples with similar results. B) Cytotoxic activity of mock- or CS1-CAR-transduced NK-92 cells (NK-92-EV and NK-92-CS1-CAR, respectively) against CD138+ primary myeloma cells from 3 of 6 patients with similar results using a standard chromium-51 release assay. E/T indicates effector cell/target cell ratio. * indicates P < 0.05. C) CD138+ primary myeloma cells were co-cultured with NK-92-EV or NK-92-CS1-CAR cells at an E/T ratio of 5:1 for 24 h. Supernatants were harvested for measurement of IFN-γ secretion using ELISA. Data shown are representative of 1 out of 3 patient samples with similar results.