Figure 6.

PTS1- and PTS2-Dependent Protein Import into Peroxisomes Is Impaired in dau Mutant Pollen.

Constructs as indicated at the left were transformed into DAU/DAU qrt/qrt plants and dau/DAU qrt/qrt plants, respectively. Homozygous lines were obtained in the T2 generation. Naturally dehisced quartet pollen grains were viewed with confocal microscopy.

(A) and (B) mCherry-PTS1 displayed punctate peroxisomal localization in DAU pollen and diffuse cytosolic localization in dau pollen.

(C) and (D) PTS2-mCherry displayed dot-like pattern in DAU pollen and a diffuse pattern in dau pollen.

(E) and (F) Peroxisome-localized mCherry-PEX7 in DAU pollen and cytosol-localized mCherry-PEX7 with some large aggregates in dau pollen.

(G) and (H) Peroxisome-localized mCherry-PEX13 in DAU pollen and weak or lack of mCherry-PEX13 in dau pollen.

(I) and (J) Peroxisome-localized PEX14-mCherry in DAU pollen and weakly diffuse PEX14-mCherry in dau pollen.

(K) and (L) Aggregation of mCherry-PEX16 in DAU pollen and weak or lack of mCherry-PEX16 in dau pollen.

Bars = 10 µm.