Figure 7.

The lhk1-1 lhk1a-1 lhk3-1 Triple Mutant Does Not Form Nodules.

(A) to (D) Shoot (A) and root (B) masses, as well as the root length (C) and the number of lateral and higher order roots (D), were scored in 28-d-old uninoculated L. japonicus (Gifu) wild-type and lhk1-1 lhk1a-1 lhk3-1 triple receptor mutant plants grown in the presence of KNO₃.

(E) and (F) Scores of infection threads (E) and nodulation events (i.e., nodule primordia and nodules) (F) are given for the L. japonicus wild type (Gifu) and the triple receptor mutant.

All values reported represent means ± 95% confidence interval (n = 10 to 35) as measured 28 d after sowing ([A] to [D]) or 7 DAI with M. loti ([E] and [F]) for nodulation counts. Asterisks denote significant differences (Student’s t test, P < 0.05).

(G) Phenotypes of the wild type and the triple mutant are shown 21 DAI.