Figure 8.

The Arabidopsis Cytokinin Receptor Mediates Nodule Organogenesis.

(A) Nodulation phenotypes of hairy roots formed on wild-type and lhk1-1 mutant shoots after transformation with the A. rhizogenes strain AR12 containing empty vector (+vector), the entire Lhk1 gene, including 5′ and 3′ untranslated regions (+Lhk1), or a vector containing the Arabidopsis AHK4 gene, including its cognate promoter and terminator (+AHK4) (see Methods).

(B) The nodulation phenotypes were scored 21 DAI with M. loti. Numbers of plants and nodules in each transformation category are given. Wild-type L. japonicus ecotype Gifu transformed with A. rhizogenes AR12 was used as a positive control. In all cases, values represent means ± 95% confidence interval.