Figure 4.

MBs Detect Endogenous Splice Variants in Living Wild-Type Cells.

(A) Protein domains and gene structure as well as the four alternative splice variants of SEF factor (At5g37055). White boxes, UTRs; red boxes, retained intronic regions; blue lines, MBs. E, exon; F, fluorophore; HIT ZF, HIT-type zinc finger; I, intron; Q, quencher.

(B) Distribution fits between the ratios of the cytoplasm and the ratios of the nuclear regions (specific and unspecific signals of transfected MBs). The left panel represents the distribution of all MBs, and the right panel represents the MB against EJ1 and intron 2 with respective histograms. Plots display significant differences between the FS (MB versus EJ1; red) and the splice variants carrying intron 2 (MB versus intron 2; green) with a P value of 2.1086e⁻⁰⁴ (by the KS test). Cells transfected with an MB targeting intron 3 (i.e., the pre-mRNA; dark gray) exhibit a significant difference in comparison with the FS variant (P value of 9.0444e⁻⁰⁴ by the KS test). The significance level α for all tests is 0.05.