Figure 2.

Phenotypes of Wild-Type Plants, Single Knockout Plants, and Double Knockout Plants of the Three Nonessential Plastid Ribosomal Protein Genes rps15, rpl33, and rpl36.

The plants were grown from stem cuttings on synthetic medium supplemented with Suc as carbon source.

(A) Comparison of the wild type with the single mutants ∆rps15, ∆rpl33, and ∆rpl36. While the ∆rps15 and ∆rpl33 plants display a wild-type-like phenotype, the ∆rpl36 knockout plants are pale and show strongly delayed greening (Rogalski et al., 2008; Fleischmann et al., 2011).

(B) Phenotypes of the ∆rps15/∆rpl33, ∆rps15/∆rpl36, and ∆rpl33/∆rpl36 double knockout plants. Note the strong pigment deficiency (particularly pronounced in the older leaves) in all three double mutants.