Figure 4.

Seedling Lethality of the ∆rps15/∆rpl33 Mutants and Rescue by Elevated Growth Temperatures.

Wild-type, ∆rps15, ∆rpl33, and ∆rps15/∆rpl33 plants were germinated either on soil ([A] and [C]) or on synthetic Suc-containing medium ([B] and [D]) and grown either at 22°C ([A] and [B]) or at 35°C ([C] and [D]).

(A) Plants germinated and grow on soil at 22°C. The photos were taken 29 d after sowing.

(B) Plants germinated and grown on synthetic medium at 22°C in the absence of antibiotics (w/o sel.) or in the presence of 500 µg/mL spectinomycin (Spec) or 50 µg/mL kanamycin (Kan). The photos were taken 22 d after sowing.

(C) Plants germinated and grown on soil at 35°C.

(D) Plants grown as in (B) but at 35°C. The plants grown without selection and the plants grown under spectinomycin selection were photographed 15 d after sowing, and the plants grown under kanamycin selection were photographed 41 d after sowing. Due to the low stability of kanamycin at elevated temperatures, the kanamycin-containing medium was replaced every 14 d.