Figure 5.

ATG11 Associates with Autophagic Vesicles.

(A) to (C) Rescue of the atg11-1 phenotype with the UBQ10:GFP-ATG11 transgene.

(A) Growth of wild-type, atg11-1, and UBQ10:GFP-ATG11 atg11-1 plants under nitrogen starvation. Plants were germinated and grown for 1 week on nitrogen-containing (+N) liquid medium and then transferred to either nitrogen-containing or nitrogen-deficient (−N) liquid medium for an additional 2 weeks.

(B) Senescence under an SD photoperiod. Plants were grown on soil at 21°C under an 8-h-light/16-h-dark cycle for 10 weeks.

(C) Sensitivity to fixed-carbon starvation. Seedlings were grown under an LD photoperiod (16 h of light/8 h of dark) without added Suc for 2 weeks and then placed in darkness for 13 d before returning to LD conditions for a 12-d recovery.

(D) Deposition of GFP-ATG11–containing vesicles in the vacuole by a process that requires ATG7. Wild-type and atg7-2 plants expressing UBQ10:GFP-ATG11 were grown for 6 d on nitrogen-containing medium and then transferred to nitrogen-deficient medium without or with 1 μM ConA for an additional 24 h. Root cells were imaged by confocal fluorescence microscopy. Bar = 10 μm.

(E) GFP-ATG11 colocalizes with mCherry-ATG8a in autophagic bodies. Plants stably expressing both reporters were grown for 6 d on nitrogen-containing medium and then exposed for 8 h to nitrogen-deficient medium supplemented with 1 μM ConA before confocal microscopy. Boxes outlined in the left panels were magnified three times in the right panels. Bars = 10 and 5 μm for the left and right panels, respectively.