Figure 3.

Decrease of HDAC6 expression or inhibition of its activity impairs the motility of pancreatic cancer cells. (A) PANC-1 cells transfected with control or HDAC6 siRNAs were scratched, and wound margins were imaged 0 h and 12 h later. (B) Experiments were performed as in (A), and the extent of wound closure at 12-h point was quantified by measuring the wound area compared with the initial wound area. (C) Cells transfected with control or HDAC6 siRNAs were plated onto the inside of the transwell insert, and the insert was placed in a 24-well plate containing conditional media. After 18 h, cells migrated to the underside of the insert were stained with crystal violet. (D) Experiments were performed as in (C), and the amount of migrated cells was measured and normalized to the control group. (E) Transwell migration experiments were performed as in (C), except that cells were treated with tubacin or TSA. (F) Experiments were performed as in (E), and the amount of migrated cells was measured and normalized to the control group