Figure 4.

HDAC6 overexpression enhances pancreatic cancer cell motility without affecting cell proliferation. (A) PANC-1 cells transfected with GFP, GFP-HDAC6, or GFP-HDAC6-MT were scratched, and wound margins were imaged 0 h and 9 h later. MT, catalytically inactive mutant. (B) Experiments were performed as in (A), and the extent of wound closure was quantified by measuring the wound area compared with the initial wound area. (C) Cells transfected with the indicated plasmids were plated onto the inside of the transwell insert, and the insert was placed in a 24-well plate containing conditional media. After 18 h, cells migrated to the underside of the insert were stained with crystal violet. (D) Experiments were performed as in (C), and the amount of migrated cells was measured. (E) PANC-1 cells were transfected with the indicated plasmids, and cell proliferation was examined with the MTT assay. (F) Cells were transfected with the indicated plasmids, and cell proliferation was examined by sulforhodamine B staining. (G) Cells were transfected with the indicated plasmids, and cell cycle progression was examined by flow cytometry